Культивирование (cultivation)

PILOT SUBMERGED CULTIVATION OF TRICHODERMA ASPERELLUM RNCIM F-1323 MICROMYCETE

The study is aimed at developing a pilot industrial method for producing a biofungicide based on the fungus of Trichoderma genus. The cultivation was carried out by submerging in a pilot industrial bioreactor line with an inoculation bioreactor having a total volume of 10 litres and a working bioreactor with a total volume of 100 litres. Czapek’s modified nutrient medium was used with yeast extract and molasses replacing sucrose, g/L: molasses 20; yeast extract 7; NaNO3 2; K2HPO4 1; MgSO4 0.5; KCl 0.5; FeSO4 0.01. Cultivation was carried out maintaining the following parameters: temperature 27±0.5 ºС; culture medium mixing speed 600 rpm; aeration intensity 1 L of air per 1 L of culture medium per min. The inoculum was grown on a nutrient medium of a similar composition in rocking flasks with a total and a working volume of 750 and 100 mL, respectively. Inoculated flasks were incubated using an Innova 40R shaker incubator (New Brunswick, USA) at 200 rpm for 22-24 h until a dry biomas...

2002. 01. 013. Смит Р. Олицетворение гуманистических ценностей: Ч. Шеррингтон и культивирование науки. Smith R. The embodiment of value: C. S. Sherrington and the cultivation of science // Brit. J. for the history of science. – Oxford, 2000. – Vol. 33, Pt 3, n 118. – P. 283-311